Reporter

Part:BBa_K4245134:Design

Designed by: Bria S VarnBuhler, Jared Moon, Sourav Kumar Dey, Jiahui Wu, Samie R Jaffrey, Akshaya Poonepalle, Shivaek Venkateswaran, Varnica Basavaraj, Manaswi Gorle, Sahana Ram Narayanan, Daeun Lee, Janet Standev   Group: iGEM22_Lambert_GA   (2022-09-23)

Design Notes

The Lettuce split aptamer was developed by researchers at the Weill-Cornell Medical College using the SELEX (systematic evolution of ligands by exponential enrichment) process. For the purposes of Lambert iGEM, the sequence complementary to the left portion of the target— the complement of the middle sequence (BBa_K4245131) within the Rolling Circle Product (RCP)— was added to the 3’ end of the Lettuce left sequence.


Source

The part was acquired as DNA oligos from Integrated DNA Technologies.



Modified Lettuce Left


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]